$ 717.60
Details
The 17-Hydroxyprogesterone Competitive ELISA quantitates 17-Hydroxyprogesterone in serum,plasma,urine,dried fecal extracts or cell culture medium.Principle of the methodThe 17-Hydroxyprogesterone Competitive ELISA research-use-only kit uses a specifically generated antibody to measure 17-Hydroxyprogesterone (17HO-P) and its metabolites in reconstituted buffer samples,tissue culture media samples,urine and fecal samples,or in extracted serum and plasma. This kit is not recommended for serum or plasma samples without extraction. A 17HO-P standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into the provided plate coated with a capture antibody. A 17HO-P-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to 17HO-P to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound 17HO-P-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.Rigorous validationEach manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.17-Hydroxyprogesterone (4-pregen-17-ol-3, 20-dione, 17HO-P, OHPG) is a steroid hormone from the androgen group that is found in mammals, reptiles, birds, and other vertebrates. 17HO-P is identical across all species and so this kit should measure 17HO-P from all sources. In humans, it is primarily synthesized in the adrenal glands, the gonads, testes, and placenta.
