3,3',5,5'-Tetramethylbenzidine, 25 g, Each

Details

Specifications Synonym: BM blue, Sure Blue TMB, TMB Blotting Plus, TMB substrate, TMBCAS Number: 54827-17-7Linear Formula: [-C6H2(CH3)2-4-NH2]2Molecular Weight: 240.34Beilstein Registry Number: 2808541EC Number: 259-364-6MDL Number: MFCD00007748PubChem Substance ID: 24888954Application 3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.Quality Level: PREMIUMAssay: ≥98.0% (NT)MP: 166-171 °C; 168-171 °C(lit.)Storage Temp.: 2-8°C