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Specifications Synonyms: AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl CAS No.: 9000-81-1 Analysis Note: The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel. Biochem/physiol Actions: Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine H2O to choline acetic acid. Caution: For dilute solutions (<1 mg l), add 1 mg l bsa to stabilize. solutions containing at least 1 mg protein l in dilute neutral phosphate buffer are stable at least 6 months refrigerated. Unit Definition: One unit will hydrolyze 1.0 µmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C. Physical Form: Lyophilized powder containing Tris buffer salts General Description: Molecular Weight: 280 kDa Isoelectric Point: 5.5 Extinction Coefficient: E1% = 18.0 (280 nm)Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.TypeType V-SFormlyophilized powderCompositionProtein, =50% biuretpl~5.3SolubilityTris buffer: soluble1 mg/mL (0.02 M Tris buffer, pH 7.5)Storage temp.-20°C
