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Alexa Fluor™ 350 Carboxylic Acid is a non-reactive form of Alexa Fluor™ 350 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247). Characteristics of Alexa Fluor™ 350 dye include: Laser line: 350 nm Common filter set: DAPI Excitation maxima: 346 nm Emission maxima: 442 nm More About Alexa Fluor™ 350 Dye Alexa Fluor™ 350 dye is a blue-fluorescent dye with moderate photostability and excitation that matches the 350 nm laser line. As the brightest dye available in the blue channel, it is best used for high-abundance targets like actin or tubulin. Alexa Fluor™ 350 dye is commonly used with Alexa Fluor™ 488, 594, and 647 dyes for multiplexing. Discover the Benefits of the Alexa Fluor™ Dye Series Compared to traditional dyes, Alexa Fluor™ dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor™ dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor™ Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.
