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Thermo Scientific ATP (adenosine 5'-triphosphate), Tris buffered is an extremely stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with Tris base. The nucleotide has greater than 99% purity, is free of nuclease activities and functionally tested in in vitro transcription. HighlightsGreater than 99% purity confirmed by HPLCFree of trace contaminating nucleotides, endo-, exodeoxyribonuclease and ribonuclease activitiesHigher in vitro transcription yields using Tris buffered NTPs in comparison to NTPs, titrated with NaOHHigh stability - stable for 3 years at -20°C and after multiple freeze-thaw cycles Applicationsin vitro transcriptionRNA amplificationsiRNA synthesisaRNA synthesisLigationPhosphorylationExtinction Coefficient: 15.4 x 103 M-1 x cm-1 (pH 7.0)Formula: C10H16N5O13P3