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Specifications Synonyms: beta-D-Glucuronide glucuronosohydrolaseCAS Number: 9001-45-0MDL No.: MFCD00130629Activity: secondary activity: <=7,500 units/mL sulfataseStorage: 2-8CEnzyme Commission (EC) Number: 3.2.1.31 ( BRENDA | IUBMB )UNSPSC Code: 12352204Application: β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.Biochem/physiol Actions: Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysisBiochem/physiol Actions: β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.β-Glucuronidase from Helix pomatia is a mixture of enzymes derived from the Roman snail. Optimal pH: . Glucuronidase activity: 4.5 to 5.0 . Sulfatase activity: ~6.2Inhibitors: D-glucuronic acid, D-galacturonic acid, D-glucaro-1, 4-lactonePhysical form: Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.Quality: Many beta-glucuronidases derived from mollusks also contain sulfatase activity.Some preparations have been reported to contain acid phosphatase activity.Unit Definition: One Sigma or modified Fishman unit will liberate 1.0 mug of phenolphthalein from phenolphthalein glucuronide per hr at 37 C at pH 5.0 (30 min assay).Unit Definition: Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 mumole p-nitrocatechol sulfate per hr at pH 5.0 at 37 C.RIDADR: NONH for all modes of transportWGK Germany: 3R Codes: 42/43S Codes: 23-36/37-45IOD Codes: XnSymbol: GHS08Signal Word: DangerHazard Statements: H317-H334Precautionary statements: P261-P280-P342 P311
