Biosciences Fixable Viability Stain 620, For discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications, Each

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Reacts with and covalently binds to cell-surface and intracellular aminesPermeable plasma cell membranes, such as those present in necrotic cells, allow intracellular diffusion of dye and covalent binding to higher overall concentrations of amines than in non-permeable live cellsNecrotic cells present in a typical in vitro assay label with higher levels of dye increases their fluorescence intensity 10-20 fold over viable cellsLabeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescenceExcited by Yellow-Green laser (with an excitation maximum of 523nm), but is also well-excited by the Blue laserFluorescence emission maximum of 617nmApplications: Flow cytometry, Intracellular staining