$ 105.00
Details
Specifications Synonym: Endonuclease from Serratia marcescensCAS Number: 9025-65-4EC Number: 231-791-2Enzyme Commission (EC) Number: 3.1.30.2MDL Number: MFCD00131010Application Used for the removal of nucleic acid from protein samples.Benzonase nuclease, or endonuclease from Serratia marcescens, can be used to degrade all forms of DNA and RNA while having no photolytic activity. Benzonase nuclease can also be used to prepare proteins in microcalorymetric experiments.Biochem/physiol Actions Digests native or heat-denatured DNA and RNA.Legal Information Benzonase® Nuclease is supplied by Merck KGaA and its affiliates.Benzonase is a registered trademark of Merck KGaAUnit Definition One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ?A260 of 1.0 in 30 min at pH 8.0 at 37 °C (reaction volume 2.625 ml).Physical form Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl.Recombinant: expressed in E. coliAssay: =90% (SDS-PAGE)Form: buffered aqueous glycerol solutionConcentration: =250 units/µLForeign Activity: protease, essentially freeStorage Temp.: -20°C
