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Specifications Synonym: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimideCAS Number: 23491-52-3Empirical Formula (Hill Notation): C27H28N6O · 3HCl · xH2OMolecular Weight: 561.93 (anhydrous basis)Beilstein Registry Number: 1234011MDL Number: MFCD00012678PubChem Substance ID: 24891639Application Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry. Excitation max. = 346 nm Emission max. = 460 nmBiochem/physiol Actions Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.Packaging 1 g in glass bottle; 100, 500 mg in glass bottle; 25 mg in poly bottleQuality Level : PREMIUMAssay: =98% (HPLC and TLC)Solubility: H2O: soluble20 mg/mL; phosphate buffer: precipitatesSuitability: suitable for fluorescenceStorage Temp.: -20°C