Caspase-8 Mouse Anti-Human, Unlabeled, Clone: 3-1-9, Bd, Mouse Monoclonal Antibody, Each

Details:

Caspase-8 (flice/mach-1) is a 55kda cytosolic protein with homology to the cd95/fas-associated signal transducer, fadd/mort-1, as well as to other caspase (ice/ced-3) cysteine proteases. The n-terminal region of caspase-8 contains an amino acid sequence, termed the death domain, that facilitates caspase-8-fadd direct interaction. Fadd therefore acts as an adapter molecule, allowing caspase-8 to become recruited to the cytoplasmic region of fas following receptor activation. Viral proteins (v-flips) which inhibit recruitment and activation of caspase-8 have been isolated. Caspase-8 is produced as a proenzyme (55/50kda doublet) which upon receptor aggregation is proteolytically cleaved into smaller subunits of 40/36 (doublet), and 23kda. Overexpression of caspase-8 is sufficient to induce apoptosis in certain cell lines (e.G., Mcf-7) and this phenotype is blocked by overexpression of the caspase-3 protease inhibitor, crma. The antibody recognizes both the proform of human caspase-8 (55/50kda doublet) as well as the cleaved forms which migrate at 40/36 (doublet) and 23kda in sds/page. It also immunoprecipitates the full-length human caspase-8. Full-length recombinant human caspase-8 protein was used as immunogen.Host species: mouseclone: 3-1-9isotype: igg1 κspecies reactivity: humanimmunogen: human caspase-8 full-length recombinant proteinimmunoprecipitation, western blotting