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Specifications Synonym: H2O2:H2O2 oxidoreductaseCAS Number: 9001-05-2EC Number: 232-577-1Enzyme Commission (EC) Number: 1.11.1.6MDL Number: MFCD00081483Analysis Note Protein determined by biuretApplication Catalase is used to eliminate H2O2 from enzymatic oxidation reactions.1,2Biochem/physiol Actions Activates the decomposition of hydrogen peroxide, Natural antioxidant used to study roles of reactive oxygen species in gene expression and apoptosis.Catalase catalyzes the decomposition of hydrogen peroxide into water and oxygen.Catalase from bovine liver catalyzes the decomposition of H2O2 into water and oxygen.1,2Caution Freezing of solutions is not recommended.Unit Definition One unit will decompose 1.0 µmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.Physical properties Catalase from bovine liver is a tetramer consisting of four equal subunits with a molecular weight of 60 kDa each. Each subunit contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group.Quality Level: ELITEFrom: lyophilized powderMol. Wt.: tetramer mol wt ~250 kDaContains: =0.2 wt. % ThymolIsoelectric Point: 5.4Storage Temp.: -20°CGene Information: Cow ... CAT(280743)
