CD25 Mouse anti-Human, Brilliant Violet 510, Clone: M-A251, BD, Mouse Monoclonal Antibody, Each

Details

The M-A251 monoclonal antibody specifically binds to the 55 kDa type I transmembrane glycoprotein known as the low-affinity interleukin-2 receptor alpha chain subunit (IL-2Rα). CD25 is expressed on regulatory T cells and on activated lymphocytes (T and B) and monocytes. It associates with the IL-2Rβ/CD122 and the IL-2Rγ/CD132 receptor chains to form the high-affinity IL-2R complex. CD25 expression on T and B lymphocytes is upregulated by antigenic or mitogenic stimulation. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.