Cd3Ε Hamster Anti-Mouse, Pe, Clone: 145-2c11, Bd, Hamster Monoclonal Antibody, Each

Details:

The 145-2c11 antibody reacts with the 25-kda ε chain of the t-cell receptor-associated cd3 complex, which is expressed on thymocytes, mature t lymphocytes, and nk-t cells. The cytoplasmic domain of cd3e participates in the signal transduction events which activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2c11 antibody can activate either unprimed (naive) or primed (memory/preactivated) t cells in vivo or in vitro, in the presence of fc receptor-bearing accessory cells. In contrast, plate-bound 145-2c11 can activate t cells in the absence of accessory cells. Soluble 145-2c11 antibody has been reported to induce re-directed lysis of fc receptor-bearing target cells by ctl clones and can also block lysis of specific target cells by antigen-specific ctl's. Under some conditions, t-cell activation by 145-2c11 antibody has been reported to result in apoptotic cell death. The 145-2c11 antibody does not cross-react with rat leukocytes and it has been reported that pre-incubation of thymus cell suspensions at 37°c for 2-4 hours prior to staining enhances the ability of anti-cd3ε and anti-αβ tcr mabs to detect the t-cell receptor on immature thymocytes. This antibody is routinely tested by flow cytometric analysis and is compatible with intracellular staining protocols using the bd cytofix/cytoperm™ kit (cat. No. 554714). Other applications were tested at bd biosciences pharmingen during antibody development only or reported in the literature.Host species: hamsterclone: 145-2c11isotype: igg1 κspecies reactivity: mouseimmunogen: h-2kb specific cytotoxic t lymphocyte clone bm10-37flow cytometry, intracellular staining