CD36 Mouse anti-Mouse, BB515, Clone: CRF D-2712, BD, Mouse Monoclonal Antibody, Each

Details

The CRF D-2712 monoclonal antibody specifically binds to mouse and rat CD36, an 88-kDa glycoprotein member of the class B scavenger receptor family. CD36 is a multifunctional receptor that has a variety of ligands and is expressed on many cell types. Consequently, it is known by several names, including macrophage scavenger receptor, platelet glycoprotein IV, and fatty acid translocase. Cells that express CD36 include platelets, megakaryocytes, monocytes/macrophages, dendritic cells, erythroid precursors, microvascular endothelia, skeletal, cardiac, and smooth muscle cells, adipocytes, and epithelia of retina, breast, and intestine. This multi-ligand receptor functions in the clearance of apoptotic cells and abnormal erythrocytes (in malaria and sickle-cell anemia), the transport of long-chain fatty acids and oxidized low-density lipoproteins, the regulation of angiogenesis by mediating the effects of thrombospondin-1, and the mediation of coronary vasoconstriction. Crosslinking of cell-surface CD36 with plate-bound CRF D-2712 antibody promotes internalization of photoreceptor outer segment fragments by cultured retinal pigment epithelium. The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g. 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.