$ 1,008.14
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Specifications Preparation Method Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in CIRCLEGROW formulations. Chosen reagents are combined in the accurate proportions according to the formula, milled and thoroughly blended for a uniform distribution.Applications Circlegrow® is designed for rapid growth of E. coli and high yields of plasmid DNA.Product Overview At the end of log phase, cells growing in Circlegrow begin to utilize a proprietary second carbon source that becomes available on a delayed basis. Cell growth and plasmid replication continue long after cells in conventional media have reached stationary phase or expired. Bacteria commonly grow in CIRCLEGROW to a density triple that achieved in other media. The slow post-log phase growth of bacteria in Circlegrow eliminates the need for constant OD monitoring. Circlegrow cultures left overnight or over the weekend will have a much lower percentage of dead cells, and a higher percentage of plasmid DNA, than cultures prepared with any other media. The result is a higher yield of plasmid DNA in less volume with less attention.Product 'Circlegrow media(capsules) is an encapsulated growth media for growth of E.coli and extends plasmid production past log phase.Recommended Use Add 40 capsules per liter of purified water.Typical Working Concentration 40 g/LGrade:Molecular Biology Certified Yeast Growth MediumKey Applications:Rich Medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.Application Areas:Bacteria Cell Culture; Gene Expression; Transformation; Microbiological Analysis; Transfection and Protein ExpressionProduct Type:BiochemicalsCulture Media Type:Bacterial MediaKit Compatibility:Any Plasmid Purification Kit including MP's RapidPURE™, RPM™, and PhoenIX™ LinesPresentation:CapsulesFormat:CapsulesClarity (NTU):ClearpH:Please note that the pH of CIRCLEGROW liquid increases as the temperature of the solution decreases.Solubility:Soluble in water (>40 mg/mL).Storage & Handling:Store sterile CIRCLEGROW at ambient temperature (15-30°C). Powder medium is hygroscopic, therefore minimize exposure to air to prolong shelf life of media.Certifications:Molecular Biology Certified&trade