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Specifications Synonym: DNase I, Deoxyribonucleate 5'-oligonucleotido-hydrolaseCAS Number: 9003-98-9 EC Number: 232-667-0 Enzyme Commission (EC) Number: 3.1.21.1 ( BRENDA | IUBMB ) MDL number: MFCD00130918Analysis Note Protein determined by biuret.Application Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used for the identification, localization and expression of two novel human genes similar to deoxyribonuclease I. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate a new approach to obtaining high-activity RNase, DNase, cholesterolesterase, and trypsin from cattle pancreas.Preparation Note Derived from New Zealand-sourced pancreasUnit Definition One Kunitz unit will produce a ?A260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. [Mg2 ] = 4.2 mM.Physical form Contains calcium chlorideQuality Level PREMIUMtype Type IIform lyophilized powdermol wt mol wt ~31 kDapurified by chromatographycomposition Protein, ≥80%solubility 0.15 M NaCl: soluble5.0 mg/mL, clearforeign activity Chymotrypsin ≤0.01% Protease ≤0.005% RNase ≤0.01%storage temp. −20°C