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The AA4.1 antibody reacts with a 130-140kDa type I transmembrane protein expressed on immature B lymphocytes in the adult bone marrow; and on hematopoietic progenitors and stem cells in adult bone marrow, fetal liver, and embryonic yolk sac. Although staining of splenic immature/transitional B cells has been reported, we find that the antigen density is much lower in the spleen than in the bone marrow. Staining of spleen requires amplification through the use of a second step. The FITC conjugate of mAb AA4.1, while ideal for bone marrow staining, is not effective in the spleen (please see Usage comments below). It has been observed that the staining pattern of mAb 493 (Mfr. No. 550433 and 550434 for the purified and biotinylated formats, respectively) is similar to that of mAb AA4.1, that both antibodies precipitate molecules of the same molecular weight, and that staining by mAb AA4.1 is not blocked by mAb 493, suggesting that the antibodies recognize separate epitopes of the same Early B Lineage antigen.Flow Cytometry
