Calbiochem Berberine Chloride, A Highly Potent And Selective Oxysterol Ebi2 (Gpr183) Agonist (K, Each

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A highly potent and selective oxysterol ebi2 (gpr183) agonist (kd) = 450 pm in a saturation binding assay, and ec50 = 140 pm over ec50 = 2.1Nm for its enantiomer, 7β,25-ohc, in a gtp-γs binding assay). Dose-dependently suppresses forskolin-induced camp accumulation in an ebi2-expressing sk-n-mc/cre-β-galactosidase cell line (ic50 = 2nm), but not in control cells. Stimulates migration of lps-activated spleen b-cells and anti-cd3/cd28-activated cd4 t-cells in a dose-dependent manner. In addition, pharmacological inhibition of its biosynthesis in vivo by clotrimazole, a cyp7b1inhibitor, promotes the migration of adoptively transferred pre-activated b cells to the t/b boundary, mimicking the phenotype of pre-activated b cells in ebi2-deficient mice.