Calbiochem Nox1 Inhibitor, Ml171, The Nox1 Inhibitor, Ml171, Also Referenced Under Cas 6631-94-3, Controls The Biological Activity Of Nox1, Each

Details:

A highly selective, cell-permeable, and reversible 2-acetylphenothiazine that is shown to inhibit nadph oxidase-1 (nox1) (ic50 = 0.129μM, and 0.25μM) in human ht29 and hek293 cell-based assays, respectively. Unlike most other currently used nox inhibitors, it only has marginal activity on other cellular ros-producing enzymes and receptors including the other nox isoforms (ic50 = 5μm, 3μm and 5μm for nox2, nox3, and nox4, respectively) and xanthine oxidase, xo, (ic50 = 5.5μM) in hek293 cell-based assays. It is also shown to block nox1-dependent ros generation in hek293 cultures dose-dependently at concentrations up to ∽ 100nm; however, its inhibitory effect is reversed through over-expression of nox1, thereby suggesting that this compound is highly selective for nox1, with little effect against nox2-dependent ros generation (ic50 > 10μm). Qualitatively, it displays an inhibitory effect against srcyf-induced ecm-degrading invadopodia formation in dld1 human colon cancer cells.