Calbiochem PKR Inhibitor III, 7DG CAS 26927-01-5, A cell-permeable gedunin analog, Each

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A cell-permeable gedunin analog that directly targets PKR via reversible interaction inhibits PKR-mediated cellular events by disrupting PKR signaling complexes assembly, such as IκK complex and inflammasome/pyroptosome, resulting in effective blockage of LPS-induced IκBβ degradaion (20μM) and protection against anthrax toxin LT-induced caspase-1 activation as well as subsequent pyroptosis (10μM) in murine macrophage J774 cultures. PKR is also known to mediate LT-induced apoptosis via its kinase activity in LPS-primed Macrophages derived from C57BL/6 with a resistent NLRP1 allele, inhibitable by C16 (Cat. Nos. 527450 and 527451), but not by 7DG, while LT-induced pyroptosis in J774 cultures is insensitive to C16 treatment.