ET-52b( ) DNA Vector, Each

Details

The pET-52b( ) vector is designed for cloning and high-level expression of target proteins fused with the 8 aa Strep·Tag™ II coding sequence that is cleavable with human rhinovirus (HRV) 3C protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the HRV 3C protease cleavage site (LysGluValLysPheGl(down arrow)GlyPro), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. It also encodes an optional C-terminal thrombin protease cleavage site and His·Tag™ coding sequence with 10 histidines.