$ 367.20
Details
The EpiJET Bisulfite Conversion Kit is designed for simple and reliable bisulfite conversion of DNA for methylation analysis. In the bisulfite reaction all unmethylated cytosines are deaminated and converted to uracils, while methylated cytosines remain unchanged. Bisulfite converted unmethylated cytosines are detected as thymines in the following PCR. The EpiJET Bisulfite Conversion Kit integrates the thermal DNA denaturation and the bisulfite conversion reactions into one step. This step can be performed following long or short protocol, depending on the downstream application of modified DNA. The converted DNA is when bound to a membrane of a micro column for on-column desulfonation and subsequent DNA purification steps. The converted DNA is eluted in a small volume of Elution Buffer and is suitable for a number of techniques used for the methylation status analysis, including PCR, qPCR, COBRA, sequencing. HighlightsHigh conversion efficiency and specificity (≥99%)Flexibility, provided by short DNA conversion protocolPure DNA, suitable for downstream applications and long-term storage Applications Converted DNA can be used in:PCRqPCRCombined Bisulfite Restriction Analysis (COBRA)Sequencing IncludesModification ReagentModification Solution IModification Solution IIBinding Buffer for Bisulfite Conversion KitWash buffer (conc.) for Bisulfite Conversion KitDesulfonation Buffer (conc.)Elution BufferDNA Purification Micro Columns & Collection TubesHazardous: Yes Quality Control: The kit is functionally tested by analyzing bisulfite converted fully methylated (by M.SssI) Human Genomic DNA. Performance of the kit is assayed by qPCR with primers, specific for converted and unconverted DNA. The kit passes QC requirements for DNA recovery and conversion efficiency.Shelf Life: 24 MonthsShipping Condition: Refrigerated Gel PacksStorage Condition: Ambient
