FastDigest Bsp119I, Cut at TT^CGAA sites with FastDigest Bsp119I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer, Each

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Lambda DNA digested with Bsp119I, 0.7% agarose, 7 cleavage sitesFastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.Highlights100% activity of all FastDigest enzymes in the universal buffer100% buffer compatibility with downstream applicationsComplete digestion in 5-15 minutesDirect loading on gelsNo star activity176 FastDigest enzymes available