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FreeStyle CHO Expression Medium is specifically developed to support the growth and transient transfection of CHO-S cells in suspension without adaptation. This medium requires supplementation with 8 mM glutamine before use.Formulation without hypoxanthine and thymidine for use with or without dhfr systemsAbility to support high-density suspension growth and transient transfection of CHO-S cellsAnimal origin-free, protein-free and chemically defined formulationEasy use as part of the FreeStyle MAX CHO Expression System for protein productionScalability in spinner flasks and bioreactorsFormulation without hypoxanthine and thymidine for use with or without dhfr systemsGibco FreeStyle CHO Expression Medium is made without hypoxanthine and thymidine for use in dihydrofolate reductase (dhfr) amplification systemsNon-dhfr systems require the addition of HT Supplement before useAbility to support high-density suspension growth and transient transfection of CHO-S cellsGibco FreeStyle CHO cells in suspension can be cultured directly into Gibco FreeStyle CHO Expression Medium without adaptationCells adapted to other commercially available serum-free media can be subcultured directly into Gibco FreeStyle CHO Expression Medium, usually without any further adaptationCells usually require adaptation from serum-containing formulationsCells cultured in Gibco FreeStyle CHO Expression Medium can be transfected using lipid-based transfection reagents, such as FreeStyle MAX reagentAnimal origin-free, protein-free and chemically defined formulationGibco FreeStyle CHO Expression Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interestGibco chemically defined media contain no proteins, hydrolysates, or components of unknown compositionScalability in spinner flasks and bioreactorsProtein production using Gibco FreeStyle CHO Expression Medium can be scaled up in spinner flasks or bioreactorsThe appropriate spinner or impeller speed and seeding density should be optimized for each systemDepending on the impeller design and speed, it may be necessary to supplement Gibco FreeStyle CHO Expression Medium with additional Pluronic™ F-68 to avoid sheer stress in the cultureAntibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture
