GeneJET Plasmid Maxiprep Kit, Perform large-scale isolation of high-quality plasmid DNA from recombinant E. coli cultures with this kit, Each

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GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750μg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription and transfection of robust cell lines. Efficient: High yields up to 750μg of high quality plasmid DNA from 250mL of bacterial cultureHigh concentration of DNA purified: 400ng/μlConvenient: Spin column format, centrifuge or vacuum protocolsFast: Procedure takes 60min, considerably faster than using gravity flow columns Includes:Resuspension Solution, Lysis Solution, Neutralization Solution, Endotoxin Binding Reagent, Wash Solution I (concentrated), Wash Solution II (concentrated), RNase A Solution, Elution Buffer (10mM Tris-HCl, pH 8.5), GeneJET™ Maxi Purification Columns pre-assembled with collection tubes (50mL), Collection tubes (50mL) Recommended for:Transfection of robust cell lines, FastDigest™, restriction enzyme digestion, automated fluorescent sequencing, PCR, cloning, transformation,in vitro transcription.