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Specifications Synonym: GR, NAD(P)H:oxidized-glutathione oxidoreductaseCAS number: 9001-48-3Enzyme Commission (EC) Number: 1.6.4.2MDL number: MFCD00131196Preparation Note Purified by affinity chromatographyUnit Definition One unit will reduce 1.0 µmole of oxidized glutathione per min at pH 7.6 at 25 °C.Physical form Suspension in 3.6 M (NH4)2SO4, pH 7.0, containing 0.1 mM dithiothreitolBiochem/physiol Actions Glutathione reductase IGR) is a crucial flavoenzyme in the antioxidant defense system. Reduced glutathione (GSH) is used by glutathione peroxidase to detoxify hydrogen peroxide and in the process is converted to oxidized glutathione (GSSG). The GSSG is then recycled back to GSH by glutathione reductase (GR) using NADPH that is then converted to NADP . The regenerated GSH is then available to detoxify more hydrogen peroxide. The enzyme uses FAD as a cofactor. GR and glutathione peroxidase may inhibit lipid peroxidation by functioning as antioxidant enzymes in sperm. Glutathione reductase shares a structural motif with a number of other proteins including aspartyl proteases, citrate synthase, EF hands, hemoglobins, lipocalins, and a/ß hydrolases. GR is stimulated by melatonin and is reportedly irreversibly inhibited by a number of oxygen radical generating systems.Form: ammonium sulfate suspensionMol. Wt.: mol wt 118 kDaForeign Activity: G-6-PDH, 6-PGDH, and NADPH oxidase =0.01%; lipoamide dehydrogenase =0.1%Storage Temp.: 2-8°CGene Information: bakers yeast ... GLR1(856014)
