$ 592.80
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PA1-121 detects gonadotropin releasing hormone (GnRH) in mouse brain and in amphibian (Hyla cinerea) samples. PA1-121 has been successfully used in immunohistochemistry procedures. Immunohistochemical staining of GnRH in mouse embryo brain tissue using PA1-121 results in staining of neurons in the organum vasculosum of the lamina terminalis (OVLT). In immunohistochemical procedures, 4% paraformaldehyde is recommended as a fixative. The PA1-121 immunogen is a synthetic peptide corresponding to residues pyroE(22) H W S Y G L R P G-NH2(32) of mouse GnRH. This sequence is completely conserved in rat and tree shrew and is 78% conserved in zebrafish. The PA1-121 immunizing peptide (Cat. No. PEP-168) is available for use in neutralization and control experiments.Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle. Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.
