$ 217.15
Details
Specifications HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build -up of ammonia. L-alanyl-L- glutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of L- glutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4°C for longer periods. C985E60, C985E61 and C985E62 is HiGlutaXL™ Dulbecco’s Modified Eagle Medium with 4.5gms glucose per liter, L-alanyl-L- glutamine, sodium pyruvate and sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.Storage and Shelf Life: Store at 2-8 °C away from bright light. Shelf life is 18 months. Use before expiry date given on the product label.CompositionIngredientsmg/LInorganic SaltsCalcium chloride dihydrate265.000Ferric nitrate nonahydrate0.100Magnesium sulphate anhydrous97.720Potassium chloride400.000Sodium bicarbonate3700.000Sodium chloride6400.000Sodium dihydrogen phosphate anhydrous109.000Amino AcidsGlycine30.000L-Alanyl-L-Glutamine868.000L-Arginine hydrochloride84.000L-Cystine dihydrochloride62.570L-Histidine hydrochloride monohydrate42.000L-Isoleucine105.000L-Leucine105.000L-Lysine hydrochloride146.000L-Methionine30.000L-Phenylalanine66.000L-Serine42.000L-Threonine95.000L-Tryptophan16.000L-Tyrosine disodium salt103.790L-Valine94.000VitaminsCholine chloride4.000D-Ca-Pantothenate4.000Folic acid4.000Nicotinamide4.000Pyridoxal hydrochloride4.000Riboflavin0.400Thiamine hydrochloride4.000i-Inositol7.200OthersD-Glucose4500.000Phenol red sodium salt15.900Sodium pyruvate110.000 Quality ControlAppearanceRed colored, clear solution.pH7.00-7.60Osmolality in mOsm/Kg H2O320.00 - 360.00SterilityNo bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.Cultural ResponseThe growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.Endotoxin ContentNMT 5EU/ml
