$ 243.41
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Specifications HiGlutaXL™ medium contains the stabilized dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4°C for longer periods.C985G05, C985G06 and C985G07 is HiGlutaXL™ Iscove's Modified Dulbecco's Medium with L-alanyl-L-glutamine, 25mM HEPES buffer and sodium bicarbonate. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.Storage and Shelf Life Store at 2-8°C away from bright light. Shelf life is 18 months. Use before expiry date given on the product label.CompositionIngredientsmg/LInorganic SaltsCalcium chloride dihydrate219.000Magnesium sulphate anhydrous97.720Potassium chloride330.000Potassium nitrate0.076Sodium bicarbonate3204.000Sodium chloride4505.000Sodium dihydrogen phosphate, anhydrous109.000Sodium selenite0.0173Amino AcidsGlycine30.000L-Alanine25.000L-Alanyl-L-Glutamine868.000L-Arginine hydrochloride84.000L-Asparagine25.000L-Aspartic acid30.000L-Cystine dihydrochloride91.240L-Glutamic acid75.000L-Histidine hydrochloride monohydrate42.000L-Isoleucine104.800L-Leucine104.800L-Lysine hydrochloride146.200L-Methionine30.000L-Phenylalanine66.000L-Proline40.000L-Serine42.000L-Threonine95.200L-Tryptophan16.000L-Tyrosine disodium salt104.200L-Valine93.600VitaminsCholine chloride4.000D-Biotin0.013D-Ca-Pantothenate4.000Folic acid4.000Nicotinamide4.000Pyridoxal hydrochloride4.000Riboflavin0.400Thiamine hydrochloride4.000Vitamin B120.013i-Inositol7.200OthersD-Glucose4500.000HEPES Buffer5958.000Phenol red sodium salt15.000Sodium pyruvate110.000Quality ControlAppearanceOrangish red colored, clear solution.pH7.00 -7.60Osmolality in mOsm/Kg H2O280.00 -320.00SterilityNo bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.Cultural ResponseThe growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.Endotoxin ContentNMT 5EU/ml
