$ 217.15
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Specifications HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4°C for longer periods.C985F29, C985F30 and C985F31 is alpha modification of HiGlutaXL™ Minimum Essential Medium Eagle with L-alanyl- L-glutamine, sodium bicarbonate and non-essential amino acid. It is devoid of deoxyribonucleosides and ribonucleosides. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.Storage and Shelf Life Store at 2-8°C away from bright light. Shelf life is 18 months. Use before expiry date given on the product label.CompositionIngredientsmg/LInorganic SaltsCalcium chloride dihydrate265.000Magnesium sulphate anhydrous97.720Potassium chloride400.000Sodium bicarbonate2200.000Sodium chloride6800.000Sodium dihydrogen phosphate anhydrous122.000Amino AcidsGlycine50.000L-Alanine25.000L-Alanyl-L-Glutamine434.000L-Arginine hydrochloride126.000L-Asparagine monohydrate44.000L-Aspartic acid30.000L-Cysteine hydrochloride100.000L-Cystine dihydrochloride31.300L-Glutamic acid75.000L-Histidine hydrochloride monohydrate42.000L-Isoleucine52.000L-Leucine52.000L-Lysine hydrochloride72.500L-Methionine15.000L-Phenylalanine32.000L-Proline40.000L-Serine25.000L-Threonine48.000L-Tryptophan10.000L-Tyrosine disodium salt51.900L-Valine46.000VitaminsCholine chloride1.000D-Biotin0.100D-Ca-Pantothenate1.000Folic acid1.000L-Ascorbic acid50.000Nicotinamide1.000Pyridoxal hydrochloride1.000Riboflavin0.100Thiamine hydrochloride1.000Vitamin B121.360i-Inositol2.000OthersD-Glucose1000.000Lipoic acid0.200Phenol red sodium salt11.000Sodium pyruvate110.000Quality ControlAppearanceOrangish red colored, clear solution.pH7.00 -7.60Osmolality in mOsm/Kg H2O280.00 -320.00SterilityNo bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.Cultural ResponseThe growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.Endotoxin ContentNMT 5EU/ml
