$ 217.15
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Specifications HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4°C for longer periods.C985F23, C985F24 and C985F25 is HiGlutaXL™ Minimum Essential Medium with L-alanyl-L-glutamine, Hank's balanced salts and sodium bicarbonate. Hank's salt mixture is designed to equilibrate with air, hence does not require CO2 air mixture. Cells can therefore be grown in AL056G in less CO2 or CO2 free environment. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.Storage and Shelf Life Store at 2-8°C away from bright light. Shelf life is 18 months. Use before expiry date given on the product label.CompositionIngredientsmg/LInorganic SaltsCalcium chloride dihydrate185.410Magnesium sulphate anhydrous97.720Potassium chloride400.000Potassium phosphate monobasic60.000Sodium bicarbonate350.000Sodium chloride8000.000Sodium phosphate dibasic anhydrous47.800Amino AcidsL-Alanyl-L-Glutamine434.000L-Arginine hydrochloride126.000L-Cystine dihydrochloride31.300L-Histidine hydrochloride42.000L-Isoleucine52.000L-Leucine52.000L-Lysine hydrochloride72.500L-Methionine15.000L-Phenylalanine32.000L-Threonine48.000L-Tryptophan10.000L-Tyrosine disodium salt51.900L-Valine46.000VitaminsCholine chloride1.000D-Ca-Pantothenate1.000Folic acid1.000Nicotinamide1.000Pyridoxal hydrochloride1.000Riboflavin0.100Thiamine hydrochloride1.000i-Inositol2.000OthersD-Glucose1000.000Phenol red sodium salt11.000Quality ControlAppearanceRed colored, clear solution.pH7.00 -7.60Osmolality in mOsm/Kg H2O290.00 -330.00SterilityNo bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.Cultural ResponseThe growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.Endotoxin ContentNMT 5EU/ml
