$ 107.21
Details
Specifications HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4°C for longer periods.C985E90, C985E91 and C985E92 is HiGlutaXL™ Nutrient Mixture F-10 Ham with sodium bicarbonate and L-alanyl-L-glutamine. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.Storage and Shelf Life Store at 2-8°C away from bright light. Shelf life is 18 months. Use before expiry date given on the product label.CompositionIngredientsmg/LInorganic SaltsCalcium chloride dihydrate44.100Copper sulphate pentahydrate0.0025Ferric sulphate heptahydrate0.834Magnesium sulphate anhydrous74.730Potassium chloride285.000Potassium dihydrogen phosphate83.000Sodium bicarbonate1200.000Sodium chloride7400.000Sodium phosphate dibasic anhydrous153.700Zinc sulphate heptahydrate0.0288Amino AcidsGlycine7.510L-Alanine8.910L-Alanyl-L-Glutamine217.000L-Arginine hydrochloride211.000L-Asparagine anhydrous15.010L-Aspartic acid13.300L-Cysteine dihydrochloride35.130L-Glutamic acid14.700L-Histidine hydrochloride monohydrate21.000L-Isoleucine2.600L-Leucine13.100L-Lysine hydrochloride29.300L-Methionine4.480L-Phenylalanine4.960L-Proline11.500L-Serine10.500L-Threonine3.570L-Tryptophan0.600L-Tyrosine disodium salt2.610L-Valine3.500VitaminsBiotin0.024Choline chloride0.698D-Ca-Pantothenate0.715Folic acid1.320Nicotinamide0.615Pyridoxine hydrochloride0.206Riboflavin0.376Thiamine hydrochloride1.000Vitamin B121.360i-Inositol0.541OthersD-Glucose1100.000Hypoxanthine sodium salt4.080Lipoic acid0.210Phenol red sodium salt1.300Sodium pyruvate110.000Thymidine0.730 Quality ControlAppearanceOrangish red colored, clear solution.pH7.00 -7.60Osmolality in mOsm/Kg H2O285.00 -325.00SterilityNo bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.Cultural ResponseThe growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.Endotoxin ContentNMT 5EU/ml
