$ 107.78
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Specifications HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4°C for longer periods.C985E93, C985E94 and C985E95 is HiGlutaXL™ Nutrient Mixture F-12 Ham with L-alanyl-L-glutamine and sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.Storage and Shelf Life Store at 2-8°C away from bright light. Shelf life is 18 months. Use before expiry date given on the product label.CompositionIngredientsmg/LInorganic SaltsCalcium chloride dihydrate44.100Copper sulphate pentahydrate0.0025Ferric sulphate heptahydrate0.834Magnesium chloride anhydrous57.650Potassium chloride223.600Sodium bicarbonate1176.000Sodium chloride7599.000Sodium phosphate dibasic anhydrous142.040Zinc sulphate tetrahydrate0.863Amino AcidsGlycine7.500L-Alanine8.910L-Alanyl-L-Glutamine217.000L-Arginine hydrochloride210.700L-Asparagine anhydrous15.010L-Aspartic acid13.300L-Cysteine hydrochloride35.120L-Glutamic acid14.700L-Histidine hydrochloride monohydrate20.960L-Isoleucine3.940L-Leucine13.100L-Lysine hydrochloride36.500L-Methionine4.480L-Phenylalanine4.960L-Proline34.500L-Serine10.500L-Threonine11.900L-Tryptophan2.040L-Tyrosine disodium salt7.810L-Valine11.700VitaminsBiotin0.0073Choline chloride13.960D-Ca-Pantothenate0.480Folic acid1.320Nicotinamide0.037Pyridoxine hydrochloride0.062Riboflavin0.038Thiamine hydrochloride0.340Vitamin B121.360i-Inositol18.000OthersD-Glucose1801.600Hypoxanthine sodium salt4.770Linoleic acid0.084Lipoic acid0.210Phenol red sodium salt1.240Putrescine dihydrochloride0.161Sodium pyruvate110.100Thymidine0.730Quality ControlAppearanceOrangish red colored, clear solution.pH7.00 -7.60Osmolality in mOsm/Kg H2O300.00 -340.00SterilityNo bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.Cultural ResponseThe growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.Endotoxin ContentNMT 5EU/ml
