$ 243.41
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Specifications HiGlutaXL™ medium contains the stabilised dipeptide form of L-Glutamine, L-Alanyl-L-Glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build -up of ammonia. L-Alanyl-LGlutamine incorporates L-Alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-Glutamine and L-Alanine for use by the cell. The gradual release of LGlutamine obviates the need to supplement L-Glutamine frequently and allows liquid media to be stored at 4°C for longer periods.C985F10, C985F11 and C985F12 is HiGlutaXL™ RPMI-1640 medium supplemented with 25mM HEPES buffer, L-alanyl- L-glutamine and sodium bicarbonate. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37° C prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.Storage and Shelf Life Store at 2-8°C away from bright light. Shelf life is 18 months. Use before expiry date given on the product label.CompositionIngredientsmg/LInorganic SaltsCalcium nitrate tetrahydrate100.000Disodium hydrogen phosphate anhydrous800.000Magnesium sulphate anhydrous48.840Potassium chloride400.000Sodium bicarbonate2000.000Sodium chloride6000.000Amino AcidsGlycine10.000L-Alanyl-L-Glutamine446.000L-Arginine hydrochloride241.000L-Asparagine anhydrous50.000L-Aspartic acid20.000L-Cystine dihydrochloride65.200L-Glutamic acid20.000L-Histidine hydrochloride20.960L-Hydroxyproline20.000L-Isoleucine50.000L-Leucine50.000L-Lysine hydrochloride40.000L-Methionine15.000L-Phenylalanine15.000L-Proline20.000L-Serine30.000L-Threonine20.000L-Tryptophan5.000L-Tyrosine sodium salt28.830L-Valine20.000VitaminsCholine chloride3.000D-Biotin0.200D-Ca-Pantothenate0.250Folic acid1.000Niacinamide1.000Pyridoxine hydrochloride1.000Riboflavin0.200Thiamine hydrochloride1.000Vitamin B120.005i-Inositol35.000p-Amino benzoic acid (PABA)1.000OthersD-Glucose2000.000Glutathione reduced1.000HEPES Buffer5958.000Phenol red sodium salt5.300Quality ControlAppearanceOrange colored, clear solution.pH7.00 -7.60Osmolality in mOsm/Kg H2O320.00 -360.00SterilityNo bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.Cultural ResponseThe growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.Endotoxin ContentNMT 5EU/ml
