Human IGF1, Recombinant Protein, Recombinant Protein, Each

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IGF1 Recombinant Protein, RP-10931, from Invitrogen.IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer′s disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found. This is a recombinant protein suitable for use as a control in various applications. Purity: Greater than 98% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain. Dimers/Aggregates: <1% by SDS PAGE Biological Activity: Fully biologically active compared to reference standards. Specific activity is 1 x 106 IU/mg. Biological activity at 0.2-20ng/mL. Endotoxin: <0.1ng/ug Protein Content: Determined spectrophotometrically at 280nm (0.502ext. coeff), and by RP-HPLC analysis against a calibrated standard. Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20°C. Upon reconstitution, store at 4°C for 2-7 days or aliquot and store at -20°C. For long-term storage add either HSA or BSA to 0.1%. Avoid repeated freeze-thaw cycles. Reconstitute in sterile water not less than 100 ug/mL.