McCoy's 5A (Modified) Medium, Supports propagation of many types of primary cells, established cell lines, and explants from biopsy tissues, Case of 10

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McCoy's 5A (modified) Medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues. This McCoy's 5A is modified as follows: with high glucose, L-glutamine, bacto-peptone, phenol red; without sodium pyruvate, HEPES.Modification of Basal Medium 5AContains reducing agent glutathione, bacto-peptone, and a high level of glucoseIncludes Dr. Hsu's addition of Hank's salts to enable use outside CO2 incubatorRequires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS)Uses sodium bicarbonate buffer system (2.2g/L), and therefore requires 5-10% CO2 environment to maintain physiological pHSustainable packagingCell Culture, Mammalian Cell Culture