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For sixteen years, the American Institute of Nutrition Rodent Diets, AIN-76 and AIN-76A, have been used extensively around the world. Because of numerous nutritional and technical problems encountered with the diet during this period, it was revised. Two new formulations were derived:AIN-93 g for growth, pregnancy and lactationAIN-93M for adult maintenanceSome major differences in the new formulation of AIN-93 g compared with AIN-76A are as follows:7 g Soybean oil/100 g diet was substituted for 5 g corn oil/100 g diet to increase the amount of linolenic acidCornstarch was substituted for sucroseThe amount of phosphorus was reduced to help eliminate the problem of kidney calcification in female ratsL-cystine was substituted for DL-methionine as the amino acid supplement for casein, known to be deficient in the sulfur amino acidsManganese concentration was lowered to one fifth the amount in the old dietThe amounts of Vitamin E, Vitamin K and Vitamin B12 were increasedMolybdenum, silicon, fluoride, nickel, boron, lithium and vanadium were added to the mineral mixFor the AIN-93M maintenance diet:The amount of fat was lowered to 40 g/kg diet from 70 g/kg diet and the amount of casein to 140 g/kg from 200 g/kg in the AIN-93 g dietBecause of a better balance of essential nutrients, the AIN-93 diets may prove to be a better choice than AIN-93A for long-term as well as short-term studies with laboratory rodents. J. Nutr., v. 123: 1939-1951 (1993).AIN-93 g diet is available in pelleted formAIN-93 g diet is used for growth, pregnancy and lactational phases of rodent
