OPTIZYME DdeI (HpyF31), Fisher BioReagents, Each

Details

5'...C^T N A G...3'3'...G A N T^C...5'Supplied with: 10X OPTIZYME Buffer 4Conditions for 100% Activity:1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSAIncubate at 37°CEnzyme Activity in OPTIZYME buffers:Buffer 1: 20 - 50% Buffer 2: 20 - 50% Buffer 3: 20 - 50% Buffer 4: 100% Buffer 5: 20 - 50% Storage Buffer:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerolLigation and Re-cleavage:More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI.Compatible Ends: BbvCI, Bpu10I, Bpu1102I, Eco81IMethylation Effects:Dam: Never overlaps - no effectDcm: Never overlaps - no effectCpG: Never overlaps - no effectEcoKI: Never overlaps - no effectEcoBI: May overlap - effect not determined