Buffered Peptone Water (Dehydrated), Each

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Pre-enrich food samples prior to the isolation of salmonella species using thermo scientific™ oxoid™ buffered peptone water (dehydrated). Buffered peptone water is a non-selective pre-enrichment medium. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.Low numbers and/or sub-lethally injured salmonella spp. Cells may be present in a test food sample. Pre-enrichment in non-selective buffered peptone water allows the cells time to repair and multiply before being introduced into a selective culture, improving the chances of recovering salmonella species from the sample.Supports high growth and recovery rate: rich in nutrients, the medium produces high resuscitation rates and supports intense growth.Helps recovery of sub-lethally damaged salmonellae: the phosphate buffer system of the medium prevents bacterial damage due to changes in ph of the medium and helps the recovery of sub-lethally damaged salmonellae that may be sensitive to low ph.It was noted by edel and kampelmacher1 that sub-lethal injury to salmonellae may occur in many food processes. In a survey involving isolation of salmonellae from meat that had been artificially contaminated with sub-lethally injured organisms, pre-enrichment in buffered peptone water at 37°c for 18 hours before selection in brilliant green tetrathionate bile broth showed superior results compared with a direct selection method.Sadovski2 reported that, in experiments involving isolation of salmonellae from frozen vegetables, the rapid drop in ph when using lactose broth3 as a pre-enrichment medium was detrimental to the recovery of salmonellae. This was due to the enhanced sensitivity to low ph of freeze-injured salmonellae which may contaminate frozen vegetables. Pre-enrichment with buffered peptone water maintained a high ph over a period of 24 hours of incubation. The vegetable tissue has a low buffering capacity and the medium overcame this problem.Not all products are available for sale in all territories. Please inquire.Remel™ and oxoid™ products are now part of the thermo scientific brand