$ 314.40
Details
Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with T4 DNA Ligase. The resulting plasmid can be then transformed into any competent E. coli cells.HighlightsRobust and reliable exponential amplification methodNo requirements, such as special vectors, restriction sites, or methylation status for the target plasmidNo need to destroy the starting template in a separate stepPhusion Hot Start II High Fidelity DNA Polymerase minimizes unwanted secondary mutationsAmplification of large plasmids up to 10 kbHot start modification of the polymerase prevents amplification of non-specific products and unwanted degradation of primers prior to first cycle of PCRT4 DNA Ligase included in the kit; no purification steps before or after ligationCompatible with all strains of competent E. coli cell
