$ 578.40
Details
An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturationAll B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay)This all-inclusive formulation combines the lysis reagent with lysozyme, and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteriaDepending on the particular application protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.Features of the B-PER Bacterial Protein Extraction Reagent:Detergent in Tris buffer with Lysozyme and Universal NucleaseImproved cell lysis and DNA digestion, thereby releasing soluble proteins and reducing viscosity to increase yieldsRecovery of large molecular weight proteins (high MW proteins)Cost-effective equivalent to Merck Millipore Bugbuster™ Master MixMore effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applicationsThis product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.
