DNase I Solution (1 unit/µL), RNase-free, Cleave and degrade unwanted single- and double-stranded DNA, Each

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Deoxyribonuclease I (DNase I) is a single, glycosylated polypeptide that degrades unwanted single- and double-stranded DNA. The enzyme works by cleaving DNA into 5' phosphodinucleotide and small oligonucleotide fragments. DNase I is commonly added to cell lysis reagents to remove the viscosity caused by the DNA content in bacterial cell lysates or to remove the DNA templates from RNAs produced by in vitro transcription. Two grades of DNAse are offered, one sufficient for protein work and one that is useful for any application requiring the digestion of DNA in which it is crucial to avoid damage to RNA.Highlights:Degrades and removes unwanted DNA from samplesCleaves both single-stranded and double-stranded DNACompatible with Thermo Scientific Pierce Cell Lysis ReagentsReduces viscosity of bacterial lysates (protein extracts) to facilitate pipettingSupplied as 50% glycerol stocks in 10mM Tris-HCl pH 7.5, 10mM CaCl2, 10mM MgCl2Available in two convenient formats: RNase-tested to protect RNA (Part No. 89836) and convenient protein extraction grade (Part No. 90083)General information about the use of DNase I:Calcium ions are required for DNase I activity, and trace amounts of Ca may be present at high enough concentrations for DNase I to be active; however, the use of EGTA or calcium-free buffers can reduce DNase I activity to undetectable levelsHigh levels (i.e., 100mM) of monovalent ions such as Na and K will decrease DNase I activityDNase I is inactivated by heating to 65°C for 10 minutesKunitz unit: 1 Kunitz unit is the amount of enzyme required to cause an increase in absorbance at 260nm of 0.001/min/mL at 25°C in 0.1 M NaOAc, pH 5.0, due to the degradation of highly polymerized DNADegradation assay units: 1 unit is defined as the amount of enzyme required to completely degrade 1mg of plasmid DNA in 10 minutes at 37°C in 10mM Tris-HCl, pH 7.5, 50mM MgCl2, 13mM CaCl2 Specifications (Part No. 89836):Visual: Clear liquidConcentration: 1 Unit/mLActivity: ≥ 100,000 Units/mg proteinRNase Contamination: 20 Units release < 2% non-ethanol precipitable when incubated with 3H-labeled Poly(A) RNAFunctional Analysis: 1μL enzyme degrades < 5% RNA but degrades 100% DNA Specifications (Part No. 90083):Visual: Clear, colorless liquid, free of insoluble materialUnits: ≥2500 units/mLUnit definition: 1 unit is defined as the amount of enzyme required to produce an increase in absorbance at 260nm of 0.001/min/mL at 25°C of highly polymerized DNA  Elimination of viscosity caused by DNA in protein cell lysatesRemoval of DNA templates from RNAs produced by in vitro transcriptio