NeutrAvidin Protein, Maleimide-Activated, Purified NeutrAvidin protein for Western blot, ELISA or other biotin-based detection methods, Each

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Thermo Scientific™ NeutrAvidin Protein is a specially-prepared form of avidin biotin-binding protein that decreases background in Western blotting and ELISA; available items include standard HRP, AP and fluorescent conjugates for biotin-based assays.NeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified and conjugated forms of NeutrAvidin Protein provide exceptional performance in Western blot, ELISA and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin. Highlights:Near-neutral isoelectric point – pI equals 6.3, more neutral than native avidinNearly devoid of glycosylation – decreased possibility of lectin binding compared to native avidinNo RYD recognition sequence – no known off-target binding domains like streptavidinAffordable – significantly less expensive than streptavidinAvailable in multiple formats – choose purified NeutrAvidin Protein, prepared HRP and AP conjugates or activated forms for a variety of technique