$ 158.40
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Lambda DNA digested with PstI, 0.7% agarose, 28 cleavage sitesconventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.5'..C T G C A▵G...3'3'..G▵A C G T C...5'Conditions for 100% Activity1X Buffer O:50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSAIncubate at 37°CStorage BufferPstI is supplied in: 10mM Tris-HCl (pH7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerolLigation and RecleavageAfter 50-fold overdigestion with PstI, more than 95% of the DNA fragments can be ligated and recutMethylation EffectsDam: never overlaps —no effectDcm: never overlaps —no effectCpG: never overlaps —no effectEcoKI: never overlaps —no effectEcoBI: never overlaps —no effectDigestion of Agarose-embedded DNAMinimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hoursCompatible EndsAlw21I, ApaI, BseSI, Eco24I, Mph1103I, PstI, SacI, SdaI Note:Conditions of high pH, low salt, high glycerol, 8% DMSO can cause star activity (Malyguine, E., et al., Gene, 8, 163-177, 1980). Surrounding sequences: the presence of adjacent runs of G-C base pairs confers significant resistance to cleavage (Armstrong, K. and Bauer, W.R., NAR, 10, 993-1007, 1982). 100% dUTP incorporation at the recognition site reduces PstI cleavage to 25% (Glenn, T.C., et al., Biotechniques, 17, 1086-1090, 1994). PstI will not cut AGCTGCAG when methylated by AluI methyltransferase.
