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Specifications Synonym: Pancreatic Ribonuclease, RNase A, Ribonuclease I, Ribonucleate 3'-pyrimidinooligonucleotidohydrolaseCAS Number: 9001-99-4Enzyme Commission (EC) Number: 3.1.27.5Components RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0).Analysis Note Protein determined by E1%/280.Application Suitable for:RNase protection assaysRemoval of unspecifically bound RNAAnalysis of RNA sequencesHydrolysis of RNA contained in protein samplesPlasmid DNA purificationRibonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.Features and BenefitsRNase protection assaysRemove unspecifically bound RNAAnalysis of RNA sequencesHydrolyze RNA contained in protein samplesPurification of DNARNase A is an endoribonuclease that attacks at the 3’OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.Grade: For molecular biologyForm: (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)Mol. Wt.: Mol. wt. 13.7 kDa; Mol. wt. ~13,700Concentration: 1400 - 2800 U/mLForeign activity: Endonuclease and exonuclease, none detected; NICKase and DNase, none detectedShipped in: Dry IceStorage temp.: -20°C
