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RPMI 1640 Medium (Dutch modification), Originally developed to culture human leukemic cells in suspension and as monolayer, Each

49.61

Details

Roswell Park Memorial Institute (RPMI) 1640 medium has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. This RPMI is modified as follows: with phenol red, HEPES, low sodium bicarbonate; without L-glutamine.Contains reducing agent glutathione and high concentrations of vitaminsContains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle MediumVitamins inositol and choline are present in high concentrationsFormulation has Dutch modification (addition of HEPES, with sodium bicarbonate lowered to 1g/L)Contains no proteins, lipids, or growth factorsRequires supplementation, commonly with 10% Fetal Bovine Serum (FBS)Uses sodium bicarbonate buffer system (1.0g/L) and therefore requires 5-7% CO2 environment to maintain physiological pHCell Culture, Mammalian Cell CultureOrder InfoShipping Condition: Room TemperatureComplianceGibco RPMI 1640 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Additional Information

SKU 10574846
UOM Each
UNSPSC 41116134
Manufacturer Part Number 22409015