Siglec-5/Siglec-14 Mouse anti-Human, BB515, Clone: 194128, BD, Mouse Monoclonal Antibody, Each

Details

The 194128 monoclonal antibody specifically binds to Siglec-5 (Sialic acid binding Ig-like lectin 5) and Siglec-14 (Sialic acid-binding Ig-like lectin 14). Siglec-5 is also known as CD170, CD33 antigen-like 2 (CD33L2) and OBBP2 (OB binding protein-2) whereas Siglec-14 is also referred to as SIG14. Both molecules are type I transmembrane glycoproteins that belong to the SIGLEC (sialic acid binding Ig-like lectin) family within the immunoglobulin superfamily. Siglec-5 can bind to alpha-2, 3- and alpha-2, 6-linked sialic acid glycoproteins and glycolipids. It is highly expressed on neutrophils and expressed at lower levels by monocytes/macrophages and dendritic cells. Siglec-5 can function as an inhibitory receptor for some cellular responses and enhance the anti-phagocytic responses by some macrophages. Siglec-14 has 99.5% amino acid sequence homology with Siglec-5 within their first two extracellular Ig domains and has a similar ligand binding preference. Siglec-14 is expressed by cells of myeloid origin and serves as an activating receptor. The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (eg, 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.