TaqMan Reverse Transcription Reagents, For use with reverse transcriptase-PCR (RT-PCR), Each

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Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.Contain MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)Random hexamers and oligo (dT)16 included for flexibilityIndividual components provide versatility in assay set-upCompatible with one-step or two-step RT-PCR reactionsOptimal reaction temperature: 42°CFinal product size: 5kb or lessUsing TaqMan Reverse Transcription Reagents TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.Built-in normalization The TaqMan reverse transcription reagents contain a passive internal reference to normalize non-PCR-related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.Applications:PCR & Real-Time PCR, Reverse Transcriptio