X-Gluc, Effectively screen colonies with X-Gluc, a substrate for ß-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene, Each

Details

X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below). Recommended for:Detection of GUS expression in plant cells and tissues (1)Detection of infections caused by E.coliDetection of bacterial contamination in food and water samples Note:Preparation of a 0.1M stock solution in dimethylformamide or dimethylsulfoxide is recommended.